Hotspot

ndhA

SSC · 122649-124869 · 2221 bp

Back to Pachira

Pi 0.468

MarkerSeek score 77.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2221
variable_sites: 825
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGACTGTTTGGTGGCCATAA	GTTTATCCGGCCCAATGCTA	50.0	50.0	58.1	58.0	2883 bp	0.5	59.1
ndhA_p2	GGACTGTTTGGTGGCCATAA	TTTATCCGGCCCAATGCTAC	50.0	50.0	58.1	58.0	2882 bp	0.5	59.1
ndhA_p3	GGACTGTTTGGTGGCCATAA	TTATGCGAGAATGGAAGCCC	50.0	50.0	58.1	58.0	2558 bp	0.5	59.1
ndhA_p4	CGGCCCAAAGCAATTTGATT	TTATGCGAGAATGGAAGCCC	45.0	50.0	57.9	58.0	2940 bp	0.5	59.0
ndhA_p5	GGACTGTTTGGTGGCCATAA	TCAAAGCGGTTTTCCTTGGA	50.0	45.0	58.1	57.9	2504 bp	0.5	58.9