Hotspot

trnN-GUU-trnR-ACG

SSC · 132874-133484 · 611 bp

Back to Pachira

Pi 0.473

MarkerSeek score 77.60

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 611
variable_sites: 213
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CACTACGAAGAAGTTCCGGG	CCTTGTTCCTACATGACCCG	55.0	55.0	58.0	58.0	1488 bp	0.5	59.7
trnN-GUU-trnR-ACG_p2	CCACTACGAAGAAGTTCCGG	CCTTGTTCCTACATGACCCG	55.0	55.0	58.0	58.0	1489 bp	0.5	59.7
trnN-GUU-trnR-ACG_p3	CACTACGAAGAAGTTCCGGG	TGTCCCCTCCAGTCAAGAAT	55.0	50.0	58.0	58.0	1346 bp	0.5	59.6
trnN-GUU-trnR-ACG_p4	CCACTACGAAGAAGTTCCGG	TGTCCCCTCCAGTCAAGAAT	55.0	50.0	58.0	58.0	1347 bp	0.5	59.6
trnN-GUU-trnR-ACG_p5	CACTACGAAGAAGTTCCGGG	CAGCTGAGGCATCCTAACAG	55.0	55.0	58.0	58.0	1523 bp	0.5	59.5