Hotspot

rpl32-trnL-UAG

SSC · 116132-116998 · 867 bp

Back to Pachira

Pi 0.438
MarkerSeek score 77.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
867
variable_sites
274
pi
0.438
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTGAAAGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 982 bp 0.5 58.6
rpl32-trnL-UAG_p2 CGGCGTTGAAAGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 983 bp 0.5 58.6
rpl32-trnL-UAG_p3 CGGCGTTGAAAGCTTTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 992 bp 0.5 57.7
rpl32-trnL-UAG_p4 CGGCGTTGAAAGCTTTTTCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.2 57.8 991 bp 0.5 57.1
rpl32-trnL-UAG_p5 CGGCGTTGAAAGCTTTTTCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.2 57.8 990 bp 0.5 57.1