Hotspot

ndhF-rpl32

SSC · 114712-115966 · 1255 bp

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Pi 0.462

MarkerSeek score 82.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1255
variable_sites: 523
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	GGTTGAAAGCAAGGGAAGGA	50.0	50.0	58.0	58.0	1836 bp	0.5	59.9
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	GGTTGAAAGCAAGGGAAGGA	50.0	50.0	58.1	58.0	1907 bp	0.5	59.5
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	TGGGCGATGGGGAAAATAAG	50.0	50.0	58.0	57.9	1581 bp	0.5	59.4
ndhF-rpl32_p4	GGGAAGTGGCACAAAAGGTA	TGCTCCGCTATGGCTATAGT	50.0	50.0	58.0	58.1	1711 bp	0.5	59.3
ndhF-rpl32_p5	GCCCACATACGACGAAGATT	TGGGCGATGGGGAAAATAAG	50.0	50.0	58.1	57.9	1652 bp	0.5	59.0