primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ndhF	1	CCTTGTTCCTACATGACCCG	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	58.0	57.9	0.0	1.9	0.124780	112501	114711	2733-2733	0.500000	59.0
ndhF_p2	ndhF	2	GGTGACGATACTGTAGGGGA	AGACAATCGATCAGTTCCGC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.135076	112501	114711	2850-2850	0.500000	58.9
ndhF_p3	ndhF	3	TGTCCCCTCCAGTCAAGAAT	AGAGCCGGTGAATCAGAAAC	20	20	50.0	50.0	58.0	57.9	0.0	1.9	0.145397	112501	114711	2591-2591	0.500000	58.8
ndhF_p4	ndhF	4	GTCAAGTGGAAGTGCAGTGA	AGAGCCGGTGAATCAGAAAC	20	20	50.0	50.0	58.0	57.9	0.0	1.9	0.147046	112501	114711	2794-2794	0.500000	58.8
ndhF_p5	ndhF	5	CAGCTGAGGCATCCTAACAG	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	58.0	57.9	8.4	1.9	0.149777	112501	114711	2768-2768	0.500000	58.8
ndhF-rpl32_p1	ndhF-rpl32	1	GGGAAGTGGCACAAAAGGTA	GGTTGAAAGCAAGGGAAGGA	20	20	50.0	50.0	58.0	58.0	21.4	0.0	0.014917	114712	115966	1836-1836	0.500000	59.9
ndhF-rpl32_p2	ndhF-rpl32	2	GCCCACATACGACGAAGATT	GGTTGAAAGCAAGGGAAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.068691	114712	115966	1907-1907	0.500000	59.5
ndhF-rpl32_p3	ndhF-rpl32	3	GGGAAGTGGCACAAAAGGTA	TGGGCGATGGGGAAAATAAG	20	20	50.0	50.0	58.0	57.9	21.4	0.0	0.076192	114712	115966	1581-1581	0.500000	59.4
ndhF-rpl32_p4	ndhF-rpl32	4	GGGAAGTGGCACAAAAGGTA	TGCTCCGCTATGGCTATAGT	20	20	50.0	50.0	58.0	58.1	21.4	17.4	0.085563	114712	115966	1711-1711	0.500000	59.3
ndhF-rpl32_p5	ndhF-rpl32	5	GCCCACATACGACGAAGATT	TGGGCGATGGGGAAAATAAG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.129966	114712	115966	1652-1652	0.500000	59.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.173970	116132	116998	982-982	0.500000	58.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.175341	116132	116998	983-983	0.500000	58.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	14.0	0.0	0.290417	116132	116998	992-992	0.500000	57.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGGCGTTGAAAGCTTTTTCA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	58.2	57.8	14.0	0.0	0.362435	116132	116998	991-991	0.500000	57.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGGCGTTGAAAGCTTTTTCA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.2	57.8	14.0	0.0	0.362435	116132	116998	990-990	0.500000	57.1
ccsA_p1	ccsA	1	TCCTTCCCTTGCTTTCAACC	CGAAATGGGCGGAATGTCTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.056519	117178	118149	2390-2390	0.500000	59.5
ccsA_p2	ccsA	2	TCCTTCCCTTGCTTTCAACC	TGGGCGGAATGTCTATTTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.056519	117178	118149	2385-2385	0.500000	59.5
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	CGAAATGGGCGGAATGTCTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.059726	117178	118149	1859-1859	0.500000	59.5
ccsA_p4	ccsA	4	GTGAAATCGGTAGACACGCT	TGGGCGGAATGTCTATTTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.059726	117178	118149	1854-1854	0.500000	59.5
ccsA_p5	ccsA	5	TGAAATCGGTAGACACGCTG	TGGGCGGAATGTCTATTTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.061097	117178	118149	1853-1853	0.500000	59.5
ccsA-ndhD_p1	ccsA-ndhD	1	TTGGACCGTATTCGCGATTT	CGAAATGGGCGGAATGTCTA	20	20	45.0	50.0	58.0	58.1	10.9	0.0	0.064833	118150	118448	892-892	0.500000	59.5
ccsA-ndhD_p2	ccsA-ndhD	2	TTGGACCGTATTCGCGATTT	TGGGCGGAATGTCTATTTCG	20	20	45.0	50.0	58.0	58.1	10.9	0.0	0.064833	118150	118448	887-887	0.500000	59.5
ccsA-ndhD_p3	ccsA-ndhD	3	GTAAATTCCGCACTTGTGGC	CGAAATGGGCGGAATGTCTA	20	20	50.0	50.0	58.0	58.1	3.8	0.0	0.074707	118150	118448	837-837	0.500000	59.4
ccsA-ndhD_p4	ccsA-ndhD	4	GTAAATTCCGCACTTGTGGC	TGGGCGGAATGTCTATTTCG	20	20	50.0	50.0	58.0	58.1	3.8	0.0	0.074707	118150	118448	832-832	0.500000	59.4
ccsA-ndhD_p5	ccsA-ndhD	5	TTACTTGGACCGTATTCGCG	TGGGCGGAATGTCTATTTCG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139894	118150	118448	891-891	0.500000	58.9
ndhD_p1	ndhD	1	TTGGACCGTATTCGCGATTT	CAAATTGCTTCCGCTCCAAG	20	20	45.0	50.0	58.0	58.0	10.9	0.0	0.026239	118449	119951	2242-2242	0.500000	59.8
ndhD_p2	ndhD	2	GTAAATTCCGCACTTGTGGC	CAAATTGCTTCCGCTCCAAG	20	20	50.0	50.0	58.0	58.0	3.8	0.0	0.036114	118449	119951	2187-2187	0.500000	59.7
ndhD_p3	ndhD	3	TTGGACCGTATTCGCGATTT	AGATGTGAATCCGCTTGTCC	20	20	45.0	50.0	58.0	58.0	10.9	0.0	0.042775	118449	119951	2197-2197	0.500000	59.7
ndhD_p4	ndhD	4	GTAAATTCCGCACTTGTGGC	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	58.0	58.0	3.8	0.0	0.052650	118449	119951	2142-2142	0.500000	59.6
ndhD_p5	ndhD	5	TTACTTGGACCGTATTCGCG	CAAATTGCTTCCGCTCCAAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101301	118449	119951	2246-2246	0.500000	59.2
psaC_p1	psaC	1	CTTCCGCCAGCTGGATTAAT	TGGTTAGGGCCCTTATGTGT	20	20	50.0	50.0	58.0	58.3	37.2	34.0	0.350880	120082	120327	1049-1049	0.500000	57.2
psaC_p2	psaC	2	CTTCCGCCAGCTGGATTAAT	AGGGCCCTTATGTGTCTTGA	20	20	50.0	50.0	58.0	58.3	37.2	22.3	0.358302	120082	120327	1044-1044	0.500000	57.1
psaC_p3	psaC	3	CTTCCGCCAGCTGGATTAAT	GGTTAGGGCCCTTATGTGTC	20	20	50.0	55.0	58.0	57.7	37.2	34.0	0.370612	120082	120327	1048-1048	0.500000	57.0
psaC_p4	psaC	4	GCCGAGTACAGACGTTTCTT	TGGTTAGGGCCCTTATGTGT	20	20	50.0	50.0	57.9	58.3	0.0	34.0	0.385492	120082	120327	1341-1341	0.500000	56.9
psaC_p5	psaC	5	GCCGAGTACAGACGTTTCTT	AGGGCCCTTATGTGTCTTGA	20	20	50.0	50.0	57.9	58.3	0.0	22.3	0.392914	120082	120327	1336-1336	0.500000	56.9
psaC-ndhE_p1	psaC-ndhE	1	CTTCCGCCAGCTGGATTAAT	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	58.0	57.8	37.2	0.0	0.204522	120328	120574	1376-1376	0.500000	58.4
psaC-ndhE_p2	psaC-ndhE	2	GGACAAGCGGATTCACATCT	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.207232	120328	120574	977-977	0.500000	58.3
psaC-ndhE_p3	psaC-ndhE	3	CGCCAGCTGGATTAATGGAT	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	58.1	57.8	37.2	0.0	0.275803	120328	120574	1372-1372	0.500000	57.8
psaC-ndhE_p4	psaC-ndhE	4	CGAATCTCGAGTAACTGGCC	TTGCTGTGGCTCGTCAATAA	20	20	55.0	45.0	58.2	57.8	13.1	0.0	0.335944	120328	120574	1510-1510	0.500000	57.3
psaC-ndhE_p5	psaC-ndhE	5	TTCTGTTCTTGGAGCGGAAG	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	57.8	57.8	2.4	0.0	0.347226	120328	120574	939-939	0.500000	57.2
ndhG_p1	ndhG	1	ACACATAAGGGCCCTAACCA	AGAATGAGGCCTTTCTCCCT	20	20	50.0	50.0	58.3	58.0	29.0	13.1	0.345391	121109	121639	1191-1191	0.500000	57.2
ndhG_p2	ndhG	2	TCAAGACACATAAGGGCCCT	AGAATGAGGCCTTTCTCCCT	20	20	50.0	50.0	58.3	58.0	22.3	13.1	0.352813	121109	121639	1196-1196	0.500000	57.2
ndhG_p3	ndhG	3	GACACATAAGGGCCCTAACC	AGAATGAGGCCTTTCTCCCT	20	20	55.0	50.0	57.7	58.0	29.0	13.1	0.365123	121109	121639	1192-1192	0.500000	57.1
ndhG_p4	ndhG	4	ACACATAAGGGCCCTAACCA	AGGCCTTTCTCCCTTTCTCT	20	20	50.0	50.0	58.3	57.9	29.0	0.0	0.376002	121109	121639	1185-1185	0.500000	57.0
ndhG_p5	ndhG	5	TCAAGACACATAAGGGCCCT	AGGCCTTTCTCCCTTTCTCT	20	20	50.0	50.0	58.3	57.9	22.3	0.0	0.383423	121109	121639	1190-1190	0.500000	56.9
ndhG-ndhI_p1	ndhG-ndhI	1	AATCGTGTATTGGTCCAGGC	TTATGGCCACCAAACAGTCC	20	20	50.0	50.0	58.0	58.1	0.0	8.2	0.120515	121640	122062	926-926	0.500000	59.0
ndhG-ndhI_p2	ndhG-ndhI	2	AATCGTGTATTGGTCCAGGC	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.136067	121640	122062	544-544	0.500000	58.9
ndhG-ndhI_p3	ndhG-ndhI	3	CGAAGTAAGGCCATTCCCAA	TTATGGCCACCAAACAGTCC	20	20	50.0	50.0	57.9	58.1	5.0	8.2	0.204911	121640	122062	1201-1201	0.500000	58.4
ndhG-ndhI_p4	ndhG-ndhI	4	AACGAAGTAAGGCCATTCCC	TTATGGCCACCAAACAGTCC	20	20	50.0	50.0	57.9	58.1	5.0	8.2	0.206405	121640	122062	1203-1203	0.500000	58.3
ndhG-ndhI_p5	ndhG-ndhI	5	AATCGTGTATTGGTCCAGGC	AGCAGCAAGGTACATTGGTC	20	20	50.0	50.0	58.0	58.2	0.0	20.9	0.212002	121640	122062	905-905	0.500000	58.3
ndhI_p1	ndhI	1	AGGGAGAAAGGCCTCATTCT	GCGTCAGCCAATAGGGTTTA	20	20	50.0	50.0	58.0	58.0	17.9	0.0	0.054319	122063	122566	1162-1162	0.500000	59.6
ndhI_p2	ndhI	2	AATCGTGTATTGGTCCAGGC	GCGTCAGCCAATAGGGTTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.071067	122063	122566	1503-1503	0.500000	59.4
ndhI_p3	ndhI	3	AGAGAAAGGGAGAAAGGCCT	GCGTCAGCCAATAGGGTTTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.084930	122063	122566	1168-1168	0.500000	59.3
ndhI_p4	ndhI	4	AGGGAGAAAGGCCTCATTCT	TACCAGAAGCCGAGGAAGAA	20	20	50.0	50.0	58.0	58.1	17.9	0.0	0.088617	122063	122566	1091-1091	0.500000	59.3
ndhI_p5	ndhI	5	AGGGAGAAAGGCCTCATTCT	TTACCAGAAGCCGAGGAAGA	20	20	50.0	50.0	58.0	58.1	17.9	0.0	0.088617	122063	122566	1092-1092	0.500000	59.3
ndhA_p1	ndhA	1	GGACTGTTTGGTGGCCATAA	GTTTATCCGGCCCAATGCTA	20	20	50.0	50.0	58.1	58.0	9.7	0.0	0.106468	122649	124869	2883-2883	0.500000	59.1
ndhA_p2	ndhA	2	GGACTGTTTGGTGGCCATAA	TTTATCCGGCCCAATGCTAC	20	20	50.0	50.0	58.1	58.0	9.7	0.0	0.106468	122649	124869	2882-2882	0.500000	59.1
ndhA_p3	ndhA	3	GGACTGTTTGGTGGCCATAA	TTATGCGAGAATGGAAGCCC	20	20	50.0	50.0	58.1	58.0	9.7	0.0	0.114809	122649	124869	2558-2558	0.500000	59.1
ndhA_p4	ndhA	4	CGGCCCAAAGCAATTTGATT	TTATGCGAGAATGGAAGCCC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.130361	122649	124869	2940-2940	0.500000	59.0
ndhA_p5	ndhA	5	GGACTGTTTGGTGGCCATAA	TCAAAGCGGTTTTCCTTGGA	20	20	50.0	45.0	58.1	57.9	9.7	10.4	0.142178	122649	124869	2504-2504	0.500000	58.9
ndhH_p1	ndhH	1	CAACGAATCGCACGTAGAGA	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	58.1	58.0	1.2	9.9	0.114010	124871	126052	1949-1949	0.500000	59.1
ndhH_p2	ndhH	2	TCAACGAATCGCACGTAGAG	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	58.1	58.0	1.2	9.9	0.114010	124871	126052	1950-1950	0.500000	59.1
ndhH_p3	ndhH	3	CAACGAATCGCACGTAGAGA	GAAGAAAACAAGGGGTCCGT	20	20	50.0	50.0	58.1	58.0	1.2	0.0	0.131765	124871	126052	2076-2076	0.500000	58.9
ndhH_p4	ndhH	4	TCAACGAATCGCACGTAGAG	GAAGAAAACAAGGGGTCCGT	20	20	50.0	50.0	58.1	58.0	1.2	0.0	0.131765	124871	126052	2077-2077	0.500000	58.9
ndhH_p5	ndhH	5	CAACGAATCGCACGTAGAGA	CGTCAACGACTGCTGTCTTA	20	20	50.0	50.0	58.1	58.0	1.2	19.9	0.155104	124871	126052	1944-1944	0.500000	58.8
ycf1_p1	ycf1	1	AAATCAAGCTCTGCTTCCGT	AGCCCTTTTGATTGGATGGG	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.321063	127607	127726	443-443	0.500000	57.4
ycf1_p2	ycf1	2	AAATCAAGCTCTGCTTCCGT	GCCCTTTTGATTGGATGGGA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.326459	127607	127726	442-442	0.500000	57.4
ycf1_p3	ycf1	3	TTTTTACCGACAGGCGAACT	AGCCCTTTTGATTGGATGGG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.373538	127607	127726	688-688	0.500000	57.0
ycf1_p4	ycf1	4	CCGTGTTGCTCTTGTATTGC	AGCCCTTTTGATTGGATGGG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.377246	127607	127726	427-427	0.500000	57.0
ycf1_p5	ycf1	5	TTTTTACCGACAGGCGAACT	GCCCTTTTGATTGGATGGGA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.378933	127607	127726	687-687	0.500000	57.0
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	AATGGGTGACGGTATTCTGC	GGGTTACCTGCTCTTAACGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.058335	132485	132801	1190-1190	0.500000	59.5
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	CCTTCTTCCATAACCCGAGC	GGGTTACCTGCTCTTAACGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.065021	132485	132801	625-625	0.500000	59.5
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	AATGGGTGACGGTATTCTGC	AACGGTAAAAGCGAACCCTT	20	20	50.0	45.0	58.0	58.0	0.0	5.4	0.066906	132485	132801	1175-1175	0.500000	59.5
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	AATGGGTGACGGTATTCTGC	ACGGTAAAAGCGAACCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	5.4	0.066906	132485	132801	1174-1174	0.500000	59.5
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	CCTTCTTCCATAACCCGAGC	ACGGTAAAAGCGAACCCTTT	20	20	55.0	45.0	58.0	58.0	0.0	5.4	0.073592	132485	132801	609-609	0.500000	59.4
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CACTACGAAGAAGTTCCGGG	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.032006	132874	133484	1488-1488	0.500000	59.7
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCACTACGAAGAAGTTCCGG	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.032006	132874	133484	1489-1489	0.500000	59.7
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CACTACGAAGAAGTTCCGGG	TGTCCCCTCCAGTCAAGAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.052623	132874	133484	1346-1346	0.500000	59.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CCACTACGAAGAAGTTCCGG	TGTCCCCTCCAGTCAAGAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.052623	132874	133484	1347-1347	0.500000	59.6
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CACTACGAAGAAGTTCCGGG	CAGCTGAGGCATCCTAACAG	20	20	55.0	55.0	58.0	58.0	0.0	8.4	0.057002	132874	133484	1523-1523	0.500000	59.5