Hotspot

ycf1

Genome · 103096-108180 · 5085 bp

Back to Oxytropis

Pi 0.0481

MarkerSeek score 76.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5085
variable_sites: 2286
pi: 0.0481
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACATAAGAACCGTCCCCA	CTAGCCAGACGAACAGGTTC	50.0	55.0	58.1	58.0	1097-1256 bp	0.952381	85.1
ycf1_p2	AGAACCGTCCCCATTCTGTA	CTAGCCAGACGAACAGGTTC	50.0	55.0	58.1	58.0	1090-1249 bp	0.952381	85.0
ycf1_p3	AGAACCGTCCCCATTCTGTA	GACGAACAGGTTCCACAGAA	50.0	50.0	58.1	57.8	1083-1242 bp	0.952381	83.2
ycf1_p4	AGACATAAGAACCGTCCCCA	ACGGCGTCTATTGGACAAAA	50.0	45.0	58.1	57.8	1272-1431 bp	0.952381	83.0
ycf1_p5	AGAACCGTCCCCATTCTGTA	ACGGCGTCTATTGGACAAAA	50.0	45.0	58.1	57.8	1265-1424 bp	0.952381	82.9