Hotspot

clpP

Genome · 66665-67975 · 1311 bp

Back to Oxytropis

Pi 0.0579

MarkerSeek score 77.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 1311
variable_sites: 657
pi: 0.0579
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CCCTCTTAGTCCGTTCTTGC	AACAGGGTCAGAGGGATCAA	55.0	50.0	58.0	58.0	2394-2610 bp	0.952381	85.4
clpP_p2	CCCTCTTAGTCCGTTCTTGC	ACAGGGTCAGAGGGATCAAA	55.0	50.0	58.0	58.0	2393-2609 bp	0.952381	85.4
clpP_p3	CCCTCTTAGTCCGTTCTTGC	ACGAACATACCCTGTCTCCA	55.0	50.0	58.0	58.1	2426-2642 bp	0.952381	85.1
clpP_p4	CCCTCTTAGTCCGTTCTTGC	AAAAGCACAAGCCCGAAAAC	55.0	45.0	58.0	58.1	2566-2782 bp	0.952381	84.7
clpP_p5	CCCTCTTAGTCCGTTCTTGC	CAAAAAGCACAAGCCCGAAA	55.0	45.0	58.0	58.1	2568-2784 bp	0.952381	84.7