Hotspot

rpl32-ndhF

Genome · 118968-119696 · 729 bp

Back to Oxytropis

Pi 0.0447
MarkerSeek score 84.30
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
Genome
length_bp
729
variable_sites
303
pi
0.0447
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 AAGGCTTTCAATGCTGTCCA TGGAAGTGGGAGGAAAGGTA 45.0 50.0 58.0 57.6 872-906 bp 0.904762 77.0
rpl32-ndhF_p2 AGGCTTTCAATGCTGTCCAA TGGAAGTGGGAGGAAAGGTA 45.0 50.0 58.0 57.6 871-905 bp 0.904762 77.0
rpl32-ndhF_p3 AAGGCTTTCAATGCTGTCCA TTTTGTTGCCGTGGGAAAAA 45.0 40.0 58.0 57.2 923-957 bp 0.952381 76.8
rpl32-ndhF_p4 AGGCTTTCAATGCTGTCCAA TTTTGTTGCCGTGGGAAAAA 45.0 40.0 58.0 57.2 922-956 bp 0.952381 76.8
rpl32-ndhF_p5 AGGCTTTCAATGCTGTCCAA TTTTTGTTGCCGTGGGAAAA 45.0 40.0 58.0 57.2 923-957 bp 0.952381 76.8