Hotspot

trnR-ACG-trnN-GUU

Genome · 102217-102650 · 434 bp

Back to Oxytropis

Pi 0.0535
MarkerSeek score 77.30
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
Genome
length_bp
434
variable_sites
206
pi
0.0535
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGATTGGTCGTAGGTTCAA 55.0 50.0 57.8 57.6 515-532 bp 0.952381 82.0
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG TTGGTCCTCAGTAGCTCAGT 55.0 50.0 57.8 57.7 556-573 bp 0.952381 81.9
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG GTCCTCAGTAGCTCAGTGGT 55.0 55.0 57.8 58.4 553-570 bp 0.952381 80.8
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TGGTCCTCAGTAGCTCAGTG 55.0 55.0 57.8 58.4 555-572 bp 0.952381 80.8
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG GTTGGTCCTCAGTAGCTCAG 55.0 55.0 57.8 57.3 557-574 bp 0.952381 79.1