Hotspot

rpl23-trnI-CAU

Genome · 82173-82688 · 516 bp

Back to Oxytropis

Pi 0.057
MarkerSeek score 92.90
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
Genome
length_bp
516
variable_sites
226
pi
0.057
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
92.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 TCTGTCCTAGTTGATCCCGA ATTGGCGAAGTCGTAGGTTC 50.0 50.0 57.2 58.0 586-1050 bp 0.952381 82.4
rpl23-trnI-CAU_p2 TCTGTCCTAGTTGATCCCGA GGCGAAGTCGTAGGTTCAAT 50.0 50.0 57.2 58.0 583-1047 bp 0.952381 82.4
rpl23-trnI-CAU_p3 TCTGTCCTAGTTGATCCCGA GAATGGTTAAAGCGCCCAAC 50.0 50.0 57.2 58.0 611-1075 bp 0.952381 82.2
rpl23-trnI-CAU_p4 CCTAGTTGATCCCGATTCGAC ATTGGCGAAGTCGTAGGTTC 52.4 50.0 58.0 58.0 581-1045 bp 0.952381 80.9
rpl23-trnI-CAU_p5 CCTAGTTGATCCCGATTCGAC GGCGAAGTCGTAGGTTCAAT 52.4 50.0 58.0 58.0 578-1042 bp 0.952381 80.9