Hotspot

trnP-UGG-psaJ

Genome · 62984-63632 · 649 bp

Back to Oxytropis

Pi 0.0499

MarkerSeek score 91.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 649
variable_sites: 274
pi: 0.0499
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 91.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	TGCTAAAACCGCGAACCATA	45.0	45.0	57.9	57.9	620-769 bp	0.952381	86.0
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	CGGTGCCACAGAGAGATATG	45.0	55.0	57.9	57.9	587-736 bp	0.952381	85.9
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	TGCTAAAACCGCGAACCATA	45.0	45.0	58.2	57.9	621-770 bp	0.952381	85.6
trnP-UGG-psaJ_p4	TTGTACCCAAAACAAACGCG	TGCTAAAACCGCGAACCATA	45.0	45.0	57.8	57.9	628-777 bp	0.952381	85.4
trnP-UGG-psaJ_p5	AAAACAAACGCGCTACCAAG	ATAGAGTACTTGCCACCGGT	45.0	50.0	57.9	58.1	603-752 bp	0.904762	81.9