Hotspot

atpH-atpF

Genome · 47898-48540 · 643 bp

Back to Oxytropis

Pi 0.0322

MarkerSeek score 87.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 643
variable_sites: 172
pi: 0.0322
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CCGGAAGCAGAGGACAAAAT	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	785-839 bp	0.952381	83.9
atpH-atpF_p2	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	714-768 bp	0.952381	82.4
atpH-atpF_p3	CCGGAAGCAGAGGACAAAAT	CGGATGGCCAGTAACTCAAG	50.0	55.0	58.2	58.3	787-841 bp	0.952381	82.1
atpH-atpF_p4	CCGGAAGCAGAGGACAAAAT	TGGCCAGTAACTCAAGCAAA	50.0	45.0	58.2	57.6	783-837 bp	0.952381	82.0
atpH-atpF_p5	CCGGAAGCAGAGGACAAAAT	ATCAAACCCGAAACTTCCGG	50.0	50.0	58.2	58.5	807-861 bp	0.952381	81.0