Hotspot

trnL-UAA-trnT-UGU

Genome · 13909-15021 · 1113 bp

Back to Oxytropis

Pi 0.038
MarkerSeek score 88.30
Primer available yes
Label trnL-UAA-trnT-UGU

Feature Metadata

label_name
trnL-UAA-trnT-UGU
feature_type
igs
region
Genome
length_bp
1113
variable_sites
359
pi
0.038
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
88.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnT-UGU_p1 ACCAATTTCGCCATATCCCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.0 57.9 1142-1223 bp 0.952381 85.0
trnL-UAA-trnT-UGU_p2 ACCAATTTCGCCATATCCCC GAGTCGAACCGATGACCATC 50.0 55.0 58.0 58.2 1173-1254 bp 0.952381 84.6
trnL-UAA-trnT-UGU_p3 TAGCGTCTACCAATTTCGCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.1 57.9 1150-1231 bp 0.952381 84.4
trnL-UAA-trnT-UGU_p4 TAGCGTCTACCAATTTCGCC GAGTCGAACCGATGACCATC 50.0 55.0 58.1 58.2 1181-1262 bp 0.952381 84.1
trnL-UAA-trnT-UGU_p5 CCCATTGTGAATTCCAGGGT GCGATGCTCTAACCTCTGAG 50.0 55.0 57.8 57.9 1235-1316 bp 0.952381 82.9