primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	13909	15021	1142-1223	0.952381	85.0
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	ACCAATTTCGCCATATCCCC	GAGTCGAACCGATGACCATC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.163174	13909	15021	1173-1254	0.952381	84.6
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	13909	15021	1150-1231	0.952381	84.4
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GAGTCGAACCGATGACCATC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.227983	13909	15021	1181-1262	0.952381	84.1
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.8	57.9	2.3	0.0	0.369229	13909	15021	1235-1316	0.952381	82.9
trnD-GTC-psbM_p1	trnD-GTC-psbM	1	CATAGTAGAATGGCGGTCGG	CCCCGGATCATCTGTCAATT	20	20	55.0	50.0	57.9	57.4	0.0	0.7	0.711831	31077	31487	1709-1731	0.857143	75.5
trnD-GTC-psbM_p2	trnD-GTC-psbM	2	CATAGTAGAATGGCGGTCGG	TCGATGAACTGGTTACGACC	20	20	55.0	50.0	57.9	57.4	0.0	0.0	0.708131	31077	31487	1775-1797	0.809524	72.5
trnD-GTC-psbM_p3	trnD-GTC-psbM	3	AAGTATGCCCCCATCGTCTA	TCGATGAACTGGTTACGACC	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.739248	31077	31487	1753-1775	0.809524	72.2
trnD-GTC-psbM_p4	trnD-GTC-psbM	4	GAGTAATCCGTGCCGATCTC	CCCCGGATCATCTGTCAATT	20	20	55.0	50.0	57.9	57.4	0.0	0.7	0.699120	31077	31487	1221-1246	0.523810	61.7
trnD-GTC-psbM_p5	trnD-GTC-psbM	5	GAGTAATCCGTGCCGATCTC	TCGATGAACTGGTTACGACC	20	20	55.0	50.0	57.9	57.4	0.0	0.0	0.695421	31077	31487	1287-1312	0.476190	58.5
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	CTTACCACTCGGCCATATCG	TTCATTTCCAACCCCAAGCA	20	20	55.0	45.0	57.9	57.9	2.2	0.0	0.160093	33572	34620	1088-1097	0.952381	83.9
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	CCGGATTTGAACTGGGGAAA	TTCATTTCCAACCCCAAGCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.176228	33572	34620	1128-1137	0.952381	83.7
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	CTTACCACTCGGCCATATCG	TACGCCCCGATCAATGAATC	20	20	55.0	50.0	57.9	57.8	2.2	0.0	0.229811	33572	34620	1160-1169	0.952381	83.2
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	CCGGATTTGAACTGGGGAAA	TACGCCCCGATCAATGAATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.245946	33572	34620	1200-1209	0.952381	83.0
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	CTTACCACTCGGCCATATCG	AAGTTCTTCTGTTACGCCCC	20	20	55.0	50.0	57.9	57.8	2.2	0.0	0.246425	33572	34620	1172-1181	0.904762	80.3
atpH-atpF_p1	atpH-atpF	1	CCGGAAGCAGAGGACAAAAT	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	58.2	57.9	0.0	13.0	0.287475	47898	48540	785-839	0.952381	83.9
atpH-atpF_p2	atpH-atpF	2	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.532228	47898	48540	714-768	0.952381	82.4
atpH-atpF_p3	atpH-atpF	3	CCGGAAGCAGAGGACAAAAT	CGGATGGCCAGTAACTCAAG	20	20	50.0	55.0	58.2	58.3	0.0	13.0	0.516963	47898	48540	787-841	0.952381	82.1
atpH-atpF_p4	atpH-atpF	4	CCGGAAGCAGAGGACAAAAT	TGGCCAGTAACTCAAGCAAA	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.530280	47898	48540	783-837	0.952381	82.0
atpH-atpF_p5	atpH-atpF	5	CCGGAAGCAGAGGACAAAAT	ATCAAACCCGAAACTTCCGG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.643292	47898	48540	807-861	0.952381	81.0
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	TTCCGTATGTTCCTGCGATC	20	20	55.0	50.0	58.1	57.8	16.9	0.0	0.295054	53902	54680	882-1245	0.904762	82.1
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	GTATGTTCCTGCGATCCACT	20	20	55.0	50.0	58.1	57.4	16.9	0.0	0.680147	53902	54680	878-1511	0.952381	81.6
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	CGTATGTTCCTGCGATCCAC	20	20	55.0	55.0	58.1	58.8	16.9	0.0	0.866973	53902	54680	879-1512	0.952381	80.1
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GAGGTTCGAATCCTTCCGTC	AATTCCGTATGTTCCTGCGA	20	20	55.0	45.0	58.1	57.3	16.9	0.0	0.760102	53902	54680	884-1247	0.904762	78.3
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	TTCGGAGGTTCGAATCCTTC	TTCCGTATGTTCCTGCGATC	20	20	50.0	50.0	57.3	57.8	16.9	0.0	0.886426	53902	54680	886-1249	0.904762	77.3
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	TGCTAAAACCGCGAACCATA	20	20	45.0	45.0	57.9	57.9	5.7	8.2	0.210130	62984	63632	620-769	0.952381	86.0
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	AAAACAAACGCGCTACCAAG	CGGTGCCACAGAGAGATATG	20	20	45.0	55.0	57.9	57.9	5.7	0.0	0.259868	62984	63632	587-736	0.952381	85.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CAAAACAAACGCGCTACCAA	TGCTAAAACCGCGAACCATA	20	20	45.0	45.0	58.2	57.9	5.7	8.2	0.267388	62984	63632	621-770	0.952381	85.6
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	TTGTACCCAAAACAAACGCG	TGCTAAAACCGCGAACCATA	20	20	45.0	45.0	57.8	57.9	3.5	8.2	0.286545	62984	63632	628-777	0.952381	85.4
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	AAAACAAACGCGCTACCAAG	ATAGAGTACTTGCCACCGGT	20	20	45.0	50.0	57.9	58.1	5.7	0.0	0.251928	62984	63632	603-752	0.904762	81.9
clpP_p1	clpP	1	CCCTCTTAGTCCGTTCTTGC	AACAGGGTCAGAGGGATCAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.056333	66665	67975	2394-2610	0.952381	85.4
clpP_p2	clpP	2	CCCTCTTAGTCCGTTCTTGC	ACAGGGTCAGAGGGATCAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.056333	66665	67975	2393-2609	0.952381	85.4
clpP_p3	clpP	3	CCCTCTTAGTCCGTTCTTGC	ACGAACATACCCTGTCTCCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.078519	66665	67975	2426-2642	0.952381	85.1
clpP_p4	clpP	4	CCCTCTTAGTCCGTTCTTGC	AAAAGCACAAGCCCGAAAAC	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.076951	66665	67975	2566-2782	0.952381	84.7
clpP_p5	clpP	5	CCCTCTTAGTCCGTTCTTGC	CAAAAAGCACAAGCCCGAAA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.078294	66665	67975	2568-2784	0.952381	84.7
rpl2_p1	rpl2	1	TGTATTGTAAAGACGAAGAAACA	CGCTTCAACCGGGTTATTCT	23	20	30.4	50.0	54.3	58.3	0.0	13.0	6.970741	80337	81870	1662-1684	0.857143	36.1
rpl2_p2	rpl2	2	TGTATTGTAAAGACGAAGAAACAA	CGCTTCAACCGGGTTATTCT	24	20	29.2	50.0	55.0	58.3	0.0	13.0	7.227269	80337	81870	1662-1684	0.857143	36.1
rpl2_p3	rpl2	3	TGTATTGTAAAGACGAAGAAACA	ATCATTACGCTTCAACCGGG	23	20	30.4	50.0	54.3	58.3	0.0	6.3	7.041209	80337	81870	1669-1691	0.857143	36.0
rpl2_p4	rpl2	4	TTGTATTGTAAAGACGAAGAAACA	CGCTTCAACCGGGTTATTCT	24	20	29.2	50.0	55.0	58.3	0.0	13.0	7.227269	80337	81870	1663-1685	0.857143	36.0
rpl2_p5	rpl2	5	TGTATTGTAAAGACGAAGAAACA	ACGCTTCAACCGGGTTATTC	23	20	30.4	50.0	54.3	58.6	0.0	13.0	7.259033	80337	81870	1663-1685	0.857143	36.0
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	TCTGTCCTAGTTGATCCCGA	ATTGGCGAAGTCGTAGGTTC	20	20	50.0	50.0	57.2	58.0	0.0	2.3	0.826698	82173	82688	586-1050	0.952381	82.4
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	TCTGTCCTAGTTGATCCCGA	GGCGAAGTCGTAGGTTCAAT	20	20	50.0	50.0	57.2	58.0	0.0	2.3	0.826698	82173	82688	583-1047	0.952381	82.4
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	TCTGTCCTAGTTGATCCCGA	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	57.2	58.0	0.0	2.3	0.829929	82173	82688	611-1075	0.952381	82.2
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	CCTAGTTGATCCCGATTCGAC	ATTGGCGAAGTCGTAGGTTC	21	20	52.4	50.0	58.0	58.0	7.8	2.3	1.020575	82173	82688	581-1045	0.952381	80.9
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	CCTAGTTGATCCCGATTCGAC	GGCGAAGTCGTAGGTTCAAT	21	20	52.4	50.0	58.0	58.0	7.8	2.3	1.020575	82173	82688	578-1042	0.952381	80.9
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCCA	20	22	50.0	45.5	58.0	57.8	5.8	0.0	2.191054	89071	89637	624-670	0.952381	68.5
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCCA	20	22	50.0	45.5	58.0	57.8	5.8	0.0	2.192424	89071	89637	622-668	0.952381	68.5
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCC	20	22	50.0	45.5	58.0	57.8	5.8	0.0	2.192479	89071	89637	625-671	0.952381	68.5
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	CTCGCGTGTCTACCATTTCA	ACCAGGAATATCAATGAACCCC	20	22	50.0	45.5	58.0	57.8	5.8	0.0	2.193850	89071	89637	623-669	0.952381	68.5
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCCA	20	22	50.0	45.5	58.1	57.8	0.0	0.0	2.265351	89071	89637	612-658	0.952381	68.1
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	GAGGTTAAGCGTACTCTGGC	TTCGAACCGATGACTTCCAC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.150648	92901	94469	1689-1799	0.952381	81.8
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	GGTTAAGCGTACTCTGGCAA	TTCGAACCGATGACTTCCAC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.163497	92901	94469	1687-1797	0.952381	81.7
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GAGGTTAAGCGTACTCTGGC	GATGACTTCCACCACGTCAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.239919	92901	94469	1681-1791	0.952381	81.1
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GAGGTTAAGCGTACTCTGGC	ATGACTTCCACCACGTCAAG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.245933	92901	94469	1680-1790	0.952381	81.1
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	GGTTAAGCGTACTCTGGCAA	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.252768	92901	94469	1679-1789	0.952381	81.0
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.8	57.6	18.9	0.0	0.589447	102217	102650	515-532	0.952381	82.0
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TTGGTCCTCAGTAGCTCAGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	102217	102650	556-573	0.952381	81.9
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	GTCCTCAGTAGCTCAGTGGT	20	20	55.0	55.0	57.8	58.4	18.9	0.0	0.664455	102217	102650	553-570	0.952381	80.8
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	TGGTCCTCAGTAGCTCAGTG	20	20	55.0	55.0	57.8	58.4	18.9	0.0	0.665580	102217	102650	555-572	0.952381	80.8
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	GTTGGTCCTCAGTAGCTCAG	20	20	55.0	55.0	57.8	57.3	18.9	0.0	0.879733	102217	102650	557-574	0.952381	79.1
ycf1_p1	ycf1	1	AGACATAAGAACCGTCCCCA	CTAGCCAGACGAACAGGTTC	20	20	50.0	55.0	58.1	58.0	0.0	2.1	0.055346	107280	107399	1097-1256	0.952381	85.1
ycf1_p2	ycf1	2	AGAACCGTCCCCATTCTGTA	CTAGCCAGACGAACAGGTTC	20	20	50.0	55.0	58.1	58.0	0.0	2.1	0.055346	107280	107399	1090-1249	0.952381	85.0
ycf1_p3	ycf1	3	AGAACCGTCCCCATTCTGTA	GACGAACAGGTTCCACAGAA	20	20	50.0	50.0	58.1	57.8	0.0	6.6	0.287315	107280	107399	1083-1242	0.952381	83.2
ycf1_p4	ycf1	4	AGACATAAGAACCGTCCCCA	ACGGCGTCTATTGGACAAAA	20	20	50.0	45.0	58.1	57.8	0.0	6.8	0.225867	107280	107399	1272-1431	0.952381	83.0
ycf1_p5	ycf1	5	AGAACCGTCCCCATTCTGTA	ACGGCGTCTATTGGACAAAA	20	20	50.0	45.0	58.1	57.8	0.0	6.8	0.225867	107280	107399	1265-1424	0.952381	82.9
ycf1-rps15_p1	ycf1-rps15	1	TTGATACCCAGAACGGAAGC	TCAACTGATCCAGGGTTTTCT	20	21	50.0	42.9	57.6	56.8	0.0	0.0	2.562697	108181	108625	575-597	0.952381	64.2
ycf1-rps15_p2	ycf1-rps15	2	TTGATACCCAGAACGGAAGC	TGAAATTCAACTGATCCAGGGT	20	22	50.0	40.9	57.6	57.6	0.0	0.0	2.783616	108181	108625	581-603	0.952381	62.4
ycf1-rps15_p3	ycf1-rps15	3	TTGATACCCAGAACGGAAGC	TCAACTGATCCAGGGTTTTCTT	20	22	50.0	40.9	57.6	57.5	0.0	0.0	2.850069	108181	108625	575-597	0.952381	61.9
ycf1-rps15_p4	ycf1-rps15	4	TTGATACCCAGAACGGAAGC	TTCAACTGATCCAGGGTTTTCT	20	22	50.0	40.9	57.6	57.5	0.0	0.0	2.850069	108181	108625	576-598	0.952381	61.9
ycf1-rps15_p5	ycf1-rps15	5	TTGATACCCAGAACGGAAGC	ACTGATCCAGGGTTTTCTTCT	20	21	50.0	42.9	57.6	56.5	0.0	0.0	2.856517	108181	108625	572-594	0.952381	61.9
trnL-TAG-rpl32_p1	trnL-TAG-rpl32	1	TCCTAAGAGCAGCGTGTCTA	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	118136	118814	660-816	0.952381	85.0
trnL-TAG-rpl32_p2	trnL-TAG-rpl32	2	TCCTAAGAGCAGCGTGTCTA	TTGGACAGCATTGAAAGCCT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	118136	118814	661-817	0.952381	85.0
trnL-TAG-rpl32_p3	trnL-TAG-rpl32	3	AGCAGCGTGTCTACCAATTT	TGGACAGCATTGAAAGCCTT	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.185356	118136	118814	653-809	0.952381	84.5
trnL-TAG-rpl32_p4	trnL-TAG-rpl32	4	ATGGGATTGTAGAGCGGGTA	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.097462	118136	118814	1239-1389	0.952381	84.3
trnL-TAG-rpl32_p5	trnL-TAG-rpl32	5	ATGGGATTGTAGAGCGGGTA	TTGGACAGCATTGAAAGCCT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.097462	118136	118814	1240-1390	0.952381	84.3
rpl32-ndhF_p1	rpl32-ndhF	1	AAGGCTTTCAATGCTGTCCA	TGGAAGTGGGAGGAAAGGTA	20	20	45.0	50.0	58.0	57.6	0.0	0.0	0.426783	118968	119696	872-906	0.904762	77.0
rpl32-ndhF_p2	rpl32-ndhF	2	AGGCTTTCAATGCTGTCCAA	TGGAAGTGGGAGGAAAGGTA	20	20	45.0	50.0	58.0	57.6	0.0	0.0	0.426783	118968	119696	871-905	0.904762	77.0
rpl32-ndhF_p3	rpl32-ndhF	3	AAGGCTTTCAATGCTGTCCA	TTTTGTTGCCGTGGGAAAAA	20	20	45.0	40.0	58.0	57.2	0.0	0.0	0.762887	118968	119696	923-957	0.952381	76.8
rpl32-ndhF_p4	rpl32-ndhF	4	AGGCTTTCAATGCTGTCCAA	TTTTGTTGCCGTGGGAAAAA	20	20	45.0	40.0	58.0	57.2	0.0	0.0	0.762887	118968	119696	922-956	0.952381	76.8
rpl32-ndhF_p5	rpl32-ndhF	5	AGGCTTTCAATGCTGTCCAA	TTTTTGTTGCCGTGGGAAAA	20	20	45.0	40.0	58.0	57.2	0.0	0.0	0.762887	118968	119696	923-957	0.952381	76.8
ndhF_p1	ndhF	1	ACCGAACCCTTTACAAAAGAGA	CATAGAGATGGAATAGTACATTATATT	22	27	40.9	25.9	57.6	52.7	1.0	0.0	14.710146	119697	121931	2357-2383	0.952381	39.6
ndhF_p2	ndhF	2	ACCGAACCCTTTACAAAAGAG	CATAGAGATGGAATAGTACATTATATT	21	27	42.9	25.9	56.1	52.7	1.0	0.0	15.188534	119697	121931	2357-2383	0.952381	39.6
ndhF_p3	ndhF	3	ACCGAACCCTTTACAAAAGAGAT	CATAGAGATGGAATAGTACATTATATT	23	27	39.1	25.9	58.0	52.7	1.0	0.0	15.301598	119697	121931	2357-2383	0.952381	39.6
ndhF_p4	ndhF	4	AACCGAACCCTTTACAAAAGAG	CATAGAGATGGAATAGTACATTATATT	22	27	40.9	25.9	56.8	52.7	1.0	0.0	15.459533	119697	121931	2358-2384	0.952381	39.6
ndhF_p5	ndhF	5	CCGAACCCTTTACAAAAGAGA	CATAGAGATGGAATAGTACATTATATT	21	27	42.9	25.9	55.8	52.7	1.0	0.0	15.464184	119697	121931	2356-2382	0.952381	39.6