Hotspot

psbB

LSC · 73906-75432 · 1527 bp

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Pi 0.00273

MarkerSeek score 41.60

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 8
pi: 0.00273
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	AGGGGAGGTTCATAGCATAGT	AAAAAGATAAGAGAGAAATCTTGT	47.6	25.0	57.6	52.4	1632 bp	1.0	40.4
psbB_p2	AAAAGGGGAGGTTCATAGCA	AAAAAGATAAGAGAGAAATCTTGT	45.0	25.0	56.4	52.4	1635 bp	1.0	40.4
psbB_p3	AAAAAGGGGAGGTTCATAGCA	AAAAAGATAAGAGAGAAATCTTGT	42.9	25.0	57.2	52.4	1636 bp	1.0	40.4
psbB_p4	AAAGGGGAGGTTCATAGCAT	AAAAAGATAAGAGAGAAATCTTGT	45.0	25.0	56.2	52.4	1634 bp	1.0	40.4
psbB_p5	GGGGAGGTTCATAGCATAGT	AAAAAGATAAGAGAGAAATCTTGT	50.0	25.0	56.1	52.4	1631 bp	1.0	40.4