Hotspot

psaA

LSC · 40466-42718 · 2253 bp

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Pi 0.00207

MarkerSeek score 58.60

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 9
pi: 0.00207
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	AGCTAAGCCTTGGCTAAACC	TGTTGGCGGGTTTCTTTGTA	50.0	45.0	57.9	57.9	2364 bp	1.0	78.8
psaA_p2	GCTAAGCCTTGGCTAAACCT	TGTTGGCGGGTTTCTTTGTA	50.0	45.0	57.9	57.9	2363 bp	1.0	78.8
psaA_p3	AATACGACGAGTAGTGGGGT	TGTTGGCGGGTTTCTTTGTA	50.0	45.0	57.9	57.9	2388 bp	1.0	78.8
psaA_p4	GTAGTGGGGTCCTGAGCTAA	TGTTGGCGGGTTTCTTTGTA	55.0	45.0	58.1	57.9	2378 bp	1.0	78.8
psaA_p5	TAGTGGGGTCCTGAGCTAAG	TGTTGGCGGGTTTCTTTGTA	55.0	45.0	57.8	57.9	2377 bp	1.0	78.6