Hotspot

rpl20-rps12

LSC · 70212-71047 · 836 bp

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Pi 0.00638

MarkerSeek score 61.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 10
pi: 0.00638
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTCGAATGAGCTCCACGAAA	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	1004 bp	1.0	79.2
rpl20-rps12_p2	TCGAATGAGCTCCACGAAAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	1003 bp	1.0	79.2
rpl20-rps12_p3	CACGAAAGCTTGACGCAAAT	ACAGCCAATCCGAAATGTCA	45.0	45.0	58.0	57.8	991 bp	1.0	78.9
rpl20-rps12_p4	CTCGAATGAGCTCCACGAAA	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	1009 bp	1.0	78.6
rpl20-rps12_p5	TCGAATGAGCTCCACGAAAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	1008 bp	1.0	78.6