Hotspot

petA-psbJ

LSC · 63989-64822 · 834 bp

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Pi 0.0334

MarkerSeek score 64.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 45
pi: 0.0334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCTGGCATCTGTTATTTTAGCAC	ATACTCAGGGTTGGGCTCAT	43.5	50.0	58.1	58.1	950-1019 bp	1.0	59.3
petA-psbJ_p2	TCCTGGCATCTGTTATTTTAGCA	ATACTCAGGGTTGGGCTCAT	39.1	50.0	58.1	58.1	951-1020 bp	1.0	58.7
petA-psbJ_p3	CCTGGCATCTGTTATTTTAGCAC	GTTCATACTCAGGGTTGGGC	43.5	55.0	58.1	58.2	954-1023 bp	1.0	58.1
petA-psbJ_p4	CCTGGCATCTGTTATTTTAGCA	ATACTCAGGGTTGGGCTCAT	40.9	50.0	56.7	58.1	950-1019 bp	1.0	57.9
petA-psbJ_p5	TCCTGGCATCTGTTATTTTAGCA	GTTCATACTCAGGGTTGGGC	39.1	55.0	58.1	58.2	955-1024 bp	1.0	57.5