Hotspot

petN-psbM

LSC · 29515-29867 · 353 bp

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Pi 0.0152

MarkerSeek score 59.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 10
pi: 0.0152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	497-508 bp	1.0	67.5
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	503-514 bp	1.0	66.0
petN-psbM_p3	GGCTGCTTTAATGGTCGTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.3	57.0	488-499 bp	1.0	65.6
petN-psbM_p4	TCTGTCCCTCGTAGTATGGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.6	57.0	458-469 bp	1.0	65.0
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	497-508 bp	1.0	63.2