Hotspot

trnQ-UUG-psbK

LSC · 6790-7121 · 332 bp

Back to Oxyspora

Pi 0.0307

MarkerSeek score 57.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 20
pi: 0.0307
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	AAAAAGCGTAGGCCTCTGG	50.0	52.6	58.7	57.8	469-473 bp	1.0	68.6
trnQ-UUG-psbK_p2	GAATAGCGGGACCAAAACCC	AAAAAGCGTAGGCCTCTGG	55.0	52.6	58.9	57.8	478-482 bp	1.0	66.8
trnQ-UUG-psbK_p3	AAAACCCGTTGCCTTACCAC	AAAAAGCGTAGGCCTCTGG	50.0	52.6	59.0	57.8	465-469 bp	1.0	66.4
trnQ-UUG-psbK_p4	ACCAAAACCCGTTGCCTTAC	AAAAAGCGTAGGCCTCTGG	50.0	52.6	59.0	57.8	468-472 bp	1.0	66.4
trnQ-UUG-psbK_p5	CAAAACCCGTTGCCTTACCA	AAAAAGCGTAGGCCTCTGG	50.0	52.6	59.0	57.8	466-470 bp	1.0	66.4