Hotspot

rps18

LSC · 69449-69763 · 315 bp

Back to Oxyria

Pi 0.00635
MarkerSeek score 43.90
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
LSC
length_bp
315
variable_sites
2
pi
0.00635
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 AGGAATAAATAAACTAACCAAACC TGCACAAAGGCATTCAATATGA 29.2 36.4 53.2 56.9 456 bp 1.0 40.4
rps18_p2 AGGAATAAATAAACTAACCAAACC GAGCAAAACATCACTTGAATGGA 29.2 39.1 53.2 57.9 376 bp 1.0 40.4
rps18_p3 AGGAATAAATAAACTAACCAAACC AGAGCAAAACATCACTTGAATGG 29.2 39.1 53.2 57.9 377 bp 1.0 40.4
rps18_p4 AGGAATAAATAAACTAACCAAACC AGCAAAACATCACTTGAATGGA 29.2 36.4 53.2 56.8 375 bp 1.0 40.4
rps18_p5 AAGGAATAAATAAACTAACCAAACC TGCACAAAGGCATTCAATATGA 28.0 36.4 54.0 56.9 457 bp 1.0 40.4