Hotspot

rpl16

LSC · 82898-84264 · 1367 bp

Back to Oxyria

Pi 0.00223
MarkerSeek score 61.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1367
variable_sites
3
pi
0.00223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AAACAAAAACTGGCGGATGC AAAATTCGATTGACCCCCGT 45.0 45.0 58.1 57.8 1433-1451 bp 1.0 78.7
rpl16_p2 AAACAAAAACTGGCGGATGC AAATTCGATTGACCCCCGTT 45.0 45.0 58.1 57.8 1432-1450 bp 1.0 78.7
rpl16_p3 AAACAAAAACTGGCGGATGC AATTCGATTGACCCCCGTTT 45.0 45.0 58.1 57.8 1431-1449 bp 1.0 78.7
rpl16_p4 CAAAAACTGGCGGATGCTTT AAATTCGATTGACCCCCGTT 45.0 45.0 57.8 57.8 1429-1447 bp 1.0 78.6
rpl16_p5 CAAAAACTGGCGGATGCTTT AATTCGATTGACCCCCGTTT 45.0 45.0 57.8 57.8 1428-1446 bp 1.0 78.6