Hotspot

ndhA

SSC · 126148-128174 · 2027 bp

Pi 0.00395

MarkerSeek score 49.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2027
variable_sites: 8
pi: 0.00395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTCTTTTTCTTGTTTGGTACA	TGGGAGAAATTGATCGTTGA	29.2	40.0	55.8	54.7	2122 bp	1.0	40.3
ndhA_p2	TGTTTCTTTTTCTTGTTTGGTACA	TGGGAGAAATTGATCGTTGAA	29.2	38.1	55.8	55.5	2122 bp	1.0	40.3
ndhA_p3	TGTTTCTTTTTCTTGTTTGGT	TGGGAGAAATTGATCGTTGA	28.6	40.0	52.5	54.7	2122 bp	1.0	40.3
ndhA_p4	TTGTTTCTTTTTCTTGTTTGGTACA	TGGGAGAAATTGATCGTTGA	28.0	40.0	56.5	54.7	2123 bp	1.0	40.3
ndhA_p5	TGTTTCTTTTTCTTGTTTGGTACA	ATGGGAGAAATTGATCGTTGA	29.2	38.1	55.8	55.3	2123 bp	1.0	40.3