Hotspot

rpl32-trnL-UAG

SSC · 119997-120611 · 615 bp

Back to Oxyria

Pi 0.00488
MarkerSeek score 61.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
615
variable_sites
3
pi
0.00488
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGACATTCGGCAGCATTA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 751-777 bp 1.0 76.9
rpl32-trnL-UAG_p2 AGGACATTCGGCAGCATTAA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 750-776 bp 1.0 76.9
rpl32-trnL-UAG_p3 AAGGACATTCGGCAGCATTA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 752-778 bp 1.0 76.9
rpl32-trnL-UAG_p4 AGGACATTCGGCAGCATTAA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 751-777 bp 1.0 76.9
rpl32-trnL-UAG_p5 AAAGGACATTCGGCAGCATT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.5 58.0 752-778 bp 1.0 76.6