Hotspot

ndhF-rpl32

SSC · 118735-119822 · 1088 bp

Back to Oxyria

Pi 0.00551
MarkerSeek score 61.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1088
variable_sites
6
pi
0.00551
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGCTCCGATTAACAAAGGA TAATGCTGCCGAATGTCCTT 50.0 45.0 58.0 57.6 1248 bp 1.0 76.9
ndhF-rpl32_p2 CCGCTCCGATTAACAAAGGA TTAATGCTGCCGAATGTCCT 50.0 45.0 58.0 57.6 1249 bp 1.0 76.9
ndhF-rpl32_p3 CCGCTCCGATTAACAAAGGA AATGCTGCCGAATGTCCTTT 50.0 45.0 58.0 58.5 1247 bp 1.0 76.6
ndhF-rpl32_p4 CCGCTCCGATTAACAAAGGA ATGCTGCCGAATGTCCTTTT 50.0 45.0 58.0 58.5 1246 bp 1.0 76.6
ndhF-rpl32_p5 TCGGAAAAAGAAGAAGCCCC TAATGCTGCCGAATGTCCTT 50.0 45.0 58.1 57.6 1266 bp 1.0 76.3