Hotspot

rpl22-rps19

LSC · 85479-85548 · 70 bp

Back to Oxyria

Pi 0.0286
MarkerSeek score 68.90
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
70
variable_sites
2
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 ATAGGAACGTCCACGGATCT TCTAAATTTCCGGGGGCATG 50.0 50.0 57.9 57.9 230 bp 1.0 79.6
rpl22-rps19_p2 TACTCTTCGCGCTTTGTGAA TCTAAATTTCCGGGGGCATG 45.0 50.0 57.9 57.9 203 bp 1.0 79.1
rpl22-rps19_p3 TTACTCTTCGCGCTTTGTGA TCTAAATTTCCGGGGGCATG 45.0 50.0 57.9 57.9 204 bp 1.0 79.1
rpl22-rps19_p4 GAACGTCCACGGATCTGATC TCTAAATTTCCGGGGGCATG 55.0 50.0 58.2 57.9 226 bp 1.0 78.9
rpl22-rps19_p5 ATAGGAACGTCCACGGATCT GGAAGAGAGCATTTGCCTGT 50.0 50.0 57.9 58.2 301 bp 1.0 78.6