Hotspot

petD-rpoA

LSC · 79435-79833 · 399 bp

Back to Oxyria

Pi 0.0127
MarkerSeek score 62.80
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
399
variable_sites
5
pi
0.0127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 GTAGTGGCCCTTTGGTTAGG CGAGGATAATCAAGACAGGGT 55.0 47.6 57.9 56.9 569-571 bp 1.0 63.4
petD-rpoA_p2 AGTGGCCCTTTGGTTAGGTA CGAGGATAATCAAGACAGGGT 50.0 47.6 58.2 56.9 567-569 bp 1.0 62.4
petD-rpoA_p3 TAGTGGCCCTTTGGTTAGGT CGAGGATAATCAAGACAGGGT 50.0 47.6 58.2 56.9 568-570 bp 1.0 62.4
petD-rpoA_p4 CGTAGTGGCCCTTTGGTTAG CGAGGATAATCAAGACAGGGT 55.0 47.6 58.3 56.9 570-572 bp 1.0 62.3
petD-rpoA_p5 ATCCATTTCGTCGTCCAGTC CGAGGATAATCAAGACAGGGT 50.0 47.6 57.7 56.9 616-618 bp 1.0 62.0