Hotspot

clpP-psbB

LSC · 73707-74173 · 467 bp

Back to Oxyria

Pi 0.00857
MarkerSeek score 60.90
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
467
variable_sites
4
pi
0.00857
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA TGGACAGAAATCAACCGACC 50.0 50.0 58.0 57.8 611 bp 1.0 79.1
clpP-psbB_p2 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 563 bp 1.0 78.7
clpP-psbB_p3 CGCACTATACGTCAACCCAA AACCAAGGCCGTATGCATTA 50.0 45.0 58.0 57.6 633 bp 1.0 77.1
clpP-psbB_p4 CGCACTATACGTCAACCCAA ACACGATACCAAGGCAAACC 50.0 50.0 58.0 58.5 566 bp 1.0 76.8
clpP-psbB_p5 AGTTGCATCTTCATCTCCCG TGGACAGAAATCAACCGACC 50.0 50.0 57.7 57.8 592 bp 1.0 76.5