Hotspot

petG-trnW-CCA

LSC · 67113-67221 · 109 bp

Back to Oxyria

Pi 0.0183
MarkerSeek score 62.10
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
109
variable_sites
2
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 ATAGGCGTGGTGATCAGTTG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.7 58.0 213 bp 1.0 78.1
petG-trnW-CCA_p2 ATAGGCGTGGTGATCAGTTG AGAACGTGGGTCTCCAAAAC 50.0 50.0 57.7 58.0 191 bp 1.0 78.0
petG-trnW-CCA_p3 ATAGGCGTGGTGATCAGTTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.7 57.9 177 bp 1.0 77.7
petG-trnW-CCA_p4 GTGGTGATCAGTTGGACCTT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.4 58.0 207 bp 1.0 76.1
petG-trnW-CCA_p5 GTGGTGATCAGTTGGACCTT AGAACGTGGGTCTCCAAAAC 50.0 50.0 57.4 58.0 185 bp 1.0 76.0