Hotspot

psaA-ycf3

LSC · 43538-44299 · 762 bp

Back to Oxyria

Pi 0.00394
MarkerSeek score 61.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
762
variable_sites
3
pi
0.00394
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 AGGTTTTTACGGGGTCCCTA ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.0 58.0 924 bp 1.0 79.6
psaA-ycf3_p2 TGAAAAATGACCCGGTCTGG ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.1 58.0 959 bp 1.0 79.2
psaA-ycf3_p3 AGGTTTTTACGGGGTCCCTA CGATCAAGCCGCTGAGTATT 50.0 50.0 58.0 58.1 926 bp 1.0 78.8
psaA-ycf3_p4 CTGGCCCATTCCTCAAATGA ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 943 bp 1.0 78.7
psaA-ycf3_p5 TGGCCCATTCCTCAAATGAG ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 942 bp 1.0 78.7