Hotspot

trnE-UUC-trnT-GGU

LSC · 32163-33154 · 992 bp

Back to Oxyria

Pi 0.00605
MarkerSeek score 54.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
992
variable_sites
6
pi
0.00605
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AATGCACGACCAACTTCCTT GAACCGATGACTTACGCCTT 45.0 50.0 58.0 58.0 1151-1157 bp 1.0 80.2
trnE-UUC-trnT-GGU_p2 ATGCACGACCAACTTCCTTT GAACCGATGACTTACGCCTT 45.0 50.0 58.0 58.0 1150-1156 bp 1.0 80.2
trnE-UUC-trnT-GGU_p3 AATGCACGACCAACTTCCTT CCATGGCGTTACTCTACCAC 45.0 55.0 58.0 58.1 1130-1136 bp 1.0 79.8
trnE-UUC-trnT-GGU_p4 ATGCACGACCAACTTCCTTT CCATGGCGTTACTCTACCAC 45.0 55.0 58.0 58.1 1129-1135 bp 1.0 79.8
trnE-UUC-trnT-GGU_p5 ATGCACGACCAACTTCCTTT TGGCGTTACTCTACCACTGA 45.0 50.0 58.0 58.1 1126-1132 bp 1.0 79.6