Hotspot

atpH-atpI

LSC · 14349-15025 · 677 bp

Back to Oxyria

Pi 0.0104
MarkerSeek score 65.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
677
variable_sites
7
pi
0.0104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TACCTTCTACAGCTTGGCCT CAAGCGGTATTCAGGCTCTT 50.0 50.0 58.0 58.0 866-869 bp 1.0 80.7
atpH-atpI_p2 TGGTCCAATAGAAGCAAGCC CAAGCGGTATTCAGGCTCTT 50.0 50.0 57.9 58.0 823-826 bp 1.0 80.1
atpH-atpI_p3 TACCTTCTACAGCTTGGCCT TTTTGCAACTTTAGCCGCAG 50.0 45.0 58.0 57.9 844-847 bp 1.0 79.9
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA CAAGCGGTATTCAGGCTCTT 45.0 50.0 58.2 58.0 784-787 bp 1.0 79.7
atpH-atpI_p5 GCAGTACCTTGACCAACTCC CAAGCGGTATTCAGGCTCTT 55.0 50.0 58.2 58.0 843-846 bp 1.0 79.6