Hotspot

trnS-GCU-trnG-UCC

LSC · 8752-9771 · 1020 bp

Back to Oxyria

Pi 0.00686
MarkerSeek score 58.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1020
variable_sites
7
pi
0.00686
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGGAAAGAGAGGGATTCGA AGGAATCAAATCGAAGGATCCC 50.0 45.5 58.2 57.9 1179 bp 1.0 62.5
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA GGAATCAAATCGAAGGATCCCT 50.0 45.5 58.2 57.9 1178 bp 1.0 62.5
trnS-GCU-trnG-UCC_p3 CTCGTACAGCGGATTAGCAA AGGAATCAAATCGAAGGATCCC 50.0 45.5 57.8 57.9 1142 bp 1.0 62.1
trnS-GCU-trnG-UCC_p4 CTCGTACAGCGGATTAGCAA GGAATCAAATCGAAGGATCCCT 50.0 45.5 57.8 57.9 1141 bp 1.0 62.1
trnS-GCU-trnG-UCC_p5 GACTCGTACAGCGGATTAGC GGAATCAAATCGAAGGATCCCT 55.0 45.5 58.2 57.9 1143 bp 1.0 61.8