Hotspot

rps16-trnQ-UUG

LSC · 5979-7492 · 1514 bp

Back to Oxyria

Pi 0.0152
MarkerSeek score 58.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1514
variable_sites
23
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCACACCAGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1609 bp 1.0 80.2
rps16-trnQ-UUG_p2 TCCACACCAGATCATGTCCT TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.0 58.2 1639 bp 1.0 79.4
rps16-trnQ-UUG_p3 TCCACACCAGATCATGTCCT AAATATAAGTGGGGCGTGGC 50.0 50.0 58.0 58.3 1666 bp 1.0 78.3
rps16-trnQ-UUG_p4 ATCATGTCCTTCAAGTCGCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1599 bp 1.0 77.7
rps16-trnQ-UUG_p5 TCCACACCAGATCATGTCCT GTAAGGCATCGGGTTTTGGT 50.0 50.0 58.0 58.5 1640 bp 1.0 77.1