Hotspot

ycf1

SSC · 131493-133222 · 1730 bp

Back to Oxandra

Pi 0.014

MarkerSeek score 55.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 1730
variable_sites: 58
pi: 0.014
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGCCAAGGTTTCGGATAGAA	CCGGAACTTCTTCGTAGTGG	50.0	55.0	58.0	58.0	2661 bp	1.0	82.6
ycf1_p2	GGAACGCCATTATAGGTGCA	CCGGAACTTCTTCGTAGTGG	50.0	55.0	58.0	58.0	2574 bp	1.0	82.4
ycf1_p3	TGGAACGCCATTATAGGTGC	CCGGAACTTCTTCGTAGTGG	50.0	55.0	58.0	58.0	2575 bp	1.0	82.4
ycf1_p4	CGCCAAGGTTTCGGATAGAA	CCCGGAACTTCTTCGTAGTG	50.0	55.0	58.0	58.0	2662 bp	0.857143	76.4
ycf1_p5	GGAACGCCATTATAGGTGCA	CCCGGAACTTCTTCGTAGTG	50.0	55.0	58.0	58.0	2575 bp	0.857143	76.2