Hotspot

rpoA

IRb · 81687-82814 · 1128 bp

Back to Oxandra

Pi 0.0201

MarkerSeek score 60.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: IRb
length_bp: 1128
variable_sites: 44
pi: 0.0201
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AAAACCCATTTCGTCGTCCA	GAAACACTTCAATCGGCTGC	45.0	50.0	58.0	58.0	1427-1545 bp	1.0	87.3
rpoA_p2	GTAGGCCTAGCGGTTCTAGA	GAAACACTTCAATCGGCTGC	55.0	50.0	58.0	58.0	1635-1753 bp	1.0	87.1
rpoA_p3	AAAACCCATTTCGTCGTCCA	TCATGTTCAAGCGGGTTTCA	45.0	45.0	58.0	58.0	1790-1908 bp	1.0	87.0
rpoA_p4	TCCAAAACCCATTTCGTCGT	TCATGTTCAAGCGGGTTTCA	45.0	45.0	58.0	58.0	1793-1911 bp	1.0	87.0
rpoA_p5	GTAGGCCTAGCGGTTCTAGA	TCATGTTCAAGCGGGTTTCA	55.0	45.0	58.0	58.0	1998-2116 bp	1.0	86.8