Hotspot

accD

LSC · 57041-58918 · 1878 bp

Back to Oxandra

Pi 0.0151
MarkerSeek score 60.60
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1878
variable_sites
63
pi
0.0151
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.92
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 ACGAAATAGATGTATGATAATCCGC AAGCAATGGACAGTCTTGGC 36.0 50.0 57.2 58.7 1862-1970 bp 1.0 46.6
accD_p2 CGAAATAGATGTATGATAATCCGCT AAGCAATGGACAGTCTTGGC 36.0 50.0 57.0 58.7 1861-1969 bp 1.0 46.6
accD_p3 ACGAAATAGATGTATGATAATCCGC AGCAATGGACAGTCTTGGC 36.0 52.6 57.2 58.0 1861-1969 bp 1.0 46.6
accD_p4 CGAAATAGATGTATGATAATCCGC AAGCAATGGACAGTCTTGGC 37.5 50.0 55.7 58.7 1861-1969 bp 1.0 46.6
accD_p5 CGAAATAGATGTATGATAATCCGCT AGCAATGGACAGTCTTGGC 36.0 52.6 57.0 58.0 1860-1968 bp 1.0 46.6