Hotspot

ndhF-rpl32

SSC · 116481-117089 · 609 bp

Back to Oxandra

Pi 0.0171

MarkerSeek score 62.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 609
variable_sites: 27
pi: 0.0171
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.7	57.7	673-684 bp	1.0	80.8
ndhF-rpl32_p2	CTGGAAGTGGAACGAAAGGT	TACGTTTCTTTGGAACCGCC	50.0	50.0	57.7	58.8	672-683 bp	1.0	77.1
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGTA	GTACGTTTCTTTGGAACCGC	47.6	50.0	57.6	57.7	673-684 bp	1.0	71.7
ndhF-rpl32_p4	ACGAAAGGTATGATCCATGCA	GTACGTTTCTTTGGAACCGC	42.9	50.0	57.4	57.7	662-673 bp	1.0	70.5
ndhF-rpl32_p5	TGGAAGTGGAACGAAAGGTA	GTACGTTTCTTTGGAACCGC	45.0	50.0	56.4	57.7	672-683 bp	1.0	70.1