Hotspot

petA-psbJ

LSC · 65972-67131 · 1160 bp

Back to Oxandra

Pi 0.022

MarkerSeek score 64.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 61
pi: 0.022
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	CATATTCCGGATTGGGCTCA	42.9	50.0	57.2	57.4	1222-1244 bp	1.0	65.6
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	TATTCCGGATTGGGCTCATC	42.9	50.0	57.2	57.1	1220-1242 bp	1.0	63.3
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	TCATATTCCGGATTGGGCTC	42.9	50.0	57.2	57.1	1223-1245 bp	1.0	63.3
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGAA	CATATTCCGGATTGGGCTCA	40.9	50.0	57.9	57.4	1222-1244 bp	1.0	63.1
petA-psbJ_p5	TTGAGAAGGTTCAATTGTCCGA	CATATTCCGGATTGGGCTCA	40.9	50.0	57.9	57.4	1223-1245 bp	1.0	63.1