Hotspot

trnT-UGU-trnL-UAA

LSC · 48345-49242 · 898 bp

Back to Oxandra

Pi 0.026

MarkerSeek score 75.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 50
pi: 0.026
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	933-978 bp	1.0	85.9
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCAATTCC	55.0	55.0	58.2	58.2	964-1009 bp	1.0	85.5
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	945-990 bp	1.0	85.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	936-981 bp	1.0	85.2
trnT-UGU-trnL-UAA_p5	GAGTCGAACCGATGACCATC	ATCCAATCAAGTCCGTAGCG	55.0	50.0	58.2	57.8	976-1021 bp	1.0	84.8