Hotspot

trnC-GCA-petN

LSC · 28438-29496 · 1059 bp

Back to Oxandra

Pi 0.0141

MarkerSeek score 64.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1059
variable_sites: 34
pi: 0.0141
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATCCCTTTCTCCCCAGTTCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1098-1127 bp	1.0	83.5
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1148-1177 bp	1.0	83.3
trnC-GCA-petN_p3	TCCCTTTCTCCCCAGTTCAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1097-1126 bp	1.0	82.6
trnC-GCA-petN_p4	ACTGCAAATCCCTTTCTCCC	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1105-1134 bp	1.0	82.1
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1209-1238 bp	1.0	81.5