Hotspot

rps2-rpoC2

LSC · 16498-16719 · 222 bp

Back to Oxandra

Pi 0.0298

MarkerSeek score 65.00

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 17
pi: 0.0298
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GAGCCCTTATTGCAGCTGAT	GTTTTCAAGAAACCGCTCGG	50.0	50.0	58.0	58.0	792-798 bp	1.0	83.1
rps2-rpoC2_p2	GAGCCCTTATTGCAGCTGAT	TTTCACCTGGCGAGCTAATC	50.0	50.0	58.0	58.0	930-936 bp	1.0	82.8
rps2-rpoC2_p3	GCGAGATCTGGGTCACAATT	TTTCACCTGGCGAGCTAATC	50.0	50.0	58.0	58.0	1255-1261 bp	1.0	82.2
rps2-rpoC2_p4	GGTTGAGTTTCCCCGTCTTT	TTTCACCTGGCGAGCTAATC	50.0	50.0	58.0	58.0	1053-1056 bp	0.857143	75.9
rps2-rpoC2_p5	GGTTGAGTTTCCCCGTCTTT	GTTTTCAAGAAACCGCTCGG	50.0	50.0	58.0	58.0	915-918 bp	0.857143	75.9