Hotspot

rps16-trnQ-UUG

LSC · 6009-6879 · 871 bp

Back to Oxandra

Pi 0.0171

MarkerSeek score 62.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 37
pi: 0.0171
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	944-1693 bp	1.0	80.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	948-1697 bp	1.0	76.1
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	936-1685 bp	1.0	75.7
rps16-trnQ-UUG_p4	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	943-1692 bp	1.0	75.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGTTTTGGTCCCGTTATTCG	45.0	50.0	58.1	57.1	964-1713 bp	1.0	74.2