Hotspot

ycf1

SSC · 122182-127821 · 5640 bp

Pi 0.0605

MarkerSeek score 77.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 821
pi: 0.0605
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGAATGACTGAAGCATGTCT	AACCGAATTGCCCTTTGGAT	42.9	45.0	57.7	58.1	571 bp	0.222222	37.9
ycf1_p2	ACGAATGACTGAAGCATGTCT	GAAACCGAATTGCCCTTTGG	42.9	50.0	57.7	57.9	573 bp	0.222222	37.8
ycf1_p3	ACGAATGACTGAAGCATGTCT	GGAAACCGAATTGCCCTTTG	42.9	50.0	57.7	57.9	574 bp	0.222222	37.8
ycf1_p4	ACGAATGACTGAAGCATGTCT	CCGAATTGCCCTTTGGATCT	42.9	50.0	57.7	58.2	569 bp	0.222222	36.6
ycf1_p5	ACGAATGACTGAAGCATGTCT	TAAACAGTTTGGGGGTTGGG	42.9	50.0	57.7	57.9	598 bp	0.111111	33.1