Hotspot

psbE-petL

LSC · 65077-65858 · 782 bp

Back to Oxalis

Pi 0.0455
MarkerSeek score 82.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
782
variable_sites
86
pi
0.0455
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.9 58.0 1386-1438 bp 0.222222 51.4
psbE-petL_p2 TGGTCATTGGAGCCTCCTAA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 1550-1602 bp 0.222222 51.2
psbE-petL_p3 ATGGAATTCCTTGTCGGCTC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 1475-1527 bp 0.222222 51.2
psbE-petL_p4 TCGTTCGGGTTTGATTGTGT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 58.0 58.0 1708-1760 bp 0.222222 50.9
psbE-petL_p5 CGACATTTTGTTCGTTCGGG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 1719-1771 bp 0.222222 50.8