Hotspot

trnS-GCU-trnG

LSC · 7749-8469 · 721 bp

Back to Oxalis

Pi 0.0575
MarkerSeek score 82.50
Primer available yes
Label trnS-GCU-trnG

Feature Metadata

label_name
trnS-GCU-trnG
feature_type
igs
region
LSC
length_bp
721
variable_sites
110
pi
0.0575
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 764-840 bp 1.0 63.7
trnS-GCU-trnG_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 763-839 bp 1.0 63.7
trnS-GCU-trnG_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 767-843 bp 1.0 62.6
trnS-GCU-trnG_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 766-842 bp 1.0 62.6
trnS-GCU-trnG_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 765-841 bp 1.0 62.6