Hotspot

trnH-GUG-psbA

LSC · 194-540 · 347 bp

Back to Oxalis

Pi 0.0902
MarkerSeek score 71.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
347
variable_sites
71
pi
0.0902
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 TCACAATCCACTGCCTTGAG TCTAGCGGCTGTTGAAGTTC 50.0 50.0 57.8 57.9 303-423 bp 0.222222 54.5
trnH-GUG-psbA_p2 TCACAATCCACTGCCTTGAG TTCCCTCTAGATCTAGCGGC 50.0 55.0 57.8 58.1 314-434 bp 0.222222 54.3
trnH-GUG-psbA_p3 TCACAATCCACTGCCTTGAG GTTGACAGTCAAGGTCGTGT 50.0 50.0 57.8 58.1 410-530 bp 0.222222 53.8
trnH-GUG-psbA_p4 TCACAATCCACTGCCTTGAG GGTGTAGCTGGTGTATTCGG 50.0 55.0 57.8 58.1 728-848 bp 0.222222 52.6
trnH-GUG-psbA_p5 TCACAATCCACTGCCTTGAG CCCAATTGGTCAAGGAAGCT 50.0 50.0 57.8 58.1 846-966 bp 0.222222 52.3