Hotspot

rps19

LSC · 87162-87440 · 279 bp

Back to Ottelia

Pi 0.0114

MarkerSeek score 35.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 12
pi: 0.0114
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTTCTTCATAGGAACGCCCG	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1009-1068 bp	1.0	89.0
rps19_p2	TTTCTTCATAGGAACGCCCG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1010-1069 bp	1.0	89.0
rps19_p3	ACCTTTTCACAATGGGACCC	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	1168-1227 bp	1.0	88.8
rps19_p4	ACCTTTTCACAATGGGACCC	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1169-1228 bp	1.0	88.8
rps19_p5	TCGGGCTTTGAAAACAGACA	GGGTAGAGCCGGATCTAAGT	45.0	55.0	58.0	58.0	969-1028 bp	0.736842	76.8