Hotspot

ndhF-rpl32

SSC · 115068-115736 · 669 bp

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Pi 0.016
MarkerSeek score 62.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
669
variable_sites
49
pi
0.016
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCAAGCATATTGATATGTATGTTCCA AAAGCTTTAACTGCAGCCAA 34.6 40.0 57.7 56.4 753-807 bp 1.0 49.0
ndhF-rpl32_p2 CCAAGCATATTGATATGTATGTTCCA AAGCTTTAACTGCAGCCAAA 34.6 40.0 57.7 56.4 752-806 bp 1.0 49.0
ndhF-rpl32_p3 TCCAAGCATATTGATATGTATGTTCC AAGCTTTAACTGCAGCCAAA 34.6 40.0 57.4 56.4 753-807 bp 1.0 49.0
ndhF-rpl32_p4 CCAAGCATATTGATATGTATGTTCCA AAAAGCTTTAACTGCAGCCA 34.6 40.0 57.7 56.4 754-808 bp 1.0 48.9
ndhF-rpl32_p5 TCCAAGCATATTGATATGTATGTTCC AAAGCTTTAACTGCAGCCAA 34.6 40.0 57.4 56.4 754-808 bp 1.0 48.9