Hotspot

trnN-GUU-ndhF

IRb · 112220-112829 · 610 bp

Back to Ottelia

Pi 0.0102

MarkerSeek score 65.20

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 610
variable_sites: 28
pi: 0.0102
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	CGGAGGTGGACGAATTTCAT	50.0	50.0	58.2	58.0	739-1391 bp	1.0	84.8
trnN-GUU-ndhF_p2	AGCGAGCCCTTTCATTCTTC	CGGAGGTGGACGAATTTCAT	50.0	50.0	58.3	58.0	795-1447 bp	1.0	83.9
trnN-GUU-ndhF_p3	GAGCGAGCCCTTTCATTCTT	CGGAGGTGGACGAATTTCAT	50.0	50.0	58.3	58.0	796-1448 bp	1.0	83.9
trnN-GUU-ndhF_p4	GCGAGCCCTTTCATTCTTCT	CGGAGGTGGACGAATTTCAT	50.0	50.0	58.3	58.0	794-1446 bp	1.0	83.9
trnN-GUU-ndhF_p5	AATCAGTTAACAGCCGACCG	CGGAGGTGGACGAATTTCAT	50.0	50.0	58.3	58.0	720-1372 bp	1.0	83.8